Pinealon (10 mg vial)
Once daily subcutaneous, morning protocol for longevity and cellular health research.
Pinealon is a three-amino-acid Khavinson bioregulator studied in Russia for neuroprotection and circadian support. The protocol below runs short courses with months between rounds.
Quick answer: at the standard 10 mg + 1 mL reconstitution, a 2 mg dose of Pinealon draws 20 units (0.2 mL) on a U-100 insulin syringe, once daily subcutaneous, morning.
Get Pinealon → code PEPDOSE
Protocol overview
Dosing & reconstitution
Frequency: Once daily subcutaneous, morning
Typical duration: 20-day cycles, repeat every 2-3 months
Reconstitution: 10 mg vial + 1 mL bacteriostatic water = 10 mg/mL. Units below are for a U-100 insulin syringe at this concentration.
| Phase | Dose | Units per injection |
|---|---|---|
| Days 1-5 | 1 mg | 10 units (0.1 mL) |
| Days 6-14 | 1.5 mg | 15 units (0.15 mL) |
| Days 15-20 | 2 mg | 20 units (0.2 mL) |
Units = (dose ÷ concentration) × 100 on a U-100 syringe. Change the vial size or water volume and the calculator redoes all of this for you.
Track this protocol → Free account · log doses, rotate sites, never lose your history
Pinealon dosing — what the literature actually supports
We publish a schedule when there is a citable human source and we say so when there is not. Filling this space with a number nobody measured would make the page look more complete and be worth less than nothing to you.
Reconstitution and measurement
This part is arithmetic and does not depend on the evidence level. Concentration is vial size divided by the bacteriostatic water you add. Draw volume is your target amount divided by that concentration. Units are draw volume times 100, because a U-100 syringe reads 100 units per millilitre. Common vials for Pinealon: 10 mg.
- Wipe the stoppers of both vials with an alcohol swab and let them dry.
- Draw the bacteriostatic water with a sterile syringe and inject it slowly down the inside wall of the peptide vial — never straight onto the powder.
- Swirl gently until the solution is clear. Do not shake; foaming and agitation damage peptides.
- Label the vial with the date and concentration, and refrigerate at 2–8 °C protected from light.
Worked example at the calculator defaults: 10 mg + 4 mL of bacteriostatic water = 2.5 mg/mL, so on a U-100 insulin syringe 1 unit = 25 mcg of Pinealon. Change either input and the calculator redoes this for you.
Storage and stability
Supplies math
Whatever amounts your research uses, the planning arithmetic is the same. The calculator runs all of this per compound.
Supplies by protocol length
Based on the maintenance dose above (2 mg, once daily subcutaneous, morning) and a 10 mg vial reconstituted with 1 mL.
| Duration | Injections | Vials | Syringes | Bac water | Swabs |
|---|---|---|---|---|---|
| 8 weeks | 56 | 12 x 10 mg | 62 | 2 x 10 mL | 112 |
| 12 weeks | 84 | 17 x 10 mg | 93 | 2 x 10 mL | 168 |
| 16 weeks | 112 | 23 x 10 mg | 124 | 3 x 10 mL | 224 |
Syringe count includes ~10% spares. Swabs = 2 per injection (stopper + site). Vial count assumes the full maintenance dose for the entire duration.
Important notes
Practical considerations for consistency and safety.
- Use a new sterile insulin syringe for each injection and dispose in a proper sharps container.
- Rotate intramuscular injection sites (deltoid, vastus lateralis, gluteus medius) and use the appropriate needle gauge for the muscle.
- Inject slowly and steadily; wait a few seconds before withdrawing the needle to ensure complete delivery.
- Administer at the same time each day for consistent levels. Setting a daily reminder helps maintain the schedule.
- This compound lacks controlled human safety data. Start with the lowest reported amount and increase gradually while monitoring for any adverse reactions.
- The 10 mg vial size is standard for this compound. Reconstitute with 1 mL bacteriostatic water for a convenient concentration.
How Pinealon works
A tripeptide from the same Khavinson bioregulator series, associated in that literature with brain tissue and studied in rodent models of hypoxia and oxidative stress. Cell work reports that a labelled version enters the nucleus and associates with particular DNA sequences, which is the basis of the group’s claim that it acts directly on gene expression — though that association rests on fluorescence-quenching measurements rather than a direct binding assay.
Benefits & side effects
Potential benefits
- Pineal gland bioregulator
- Supports melatonin production and circadian rhythm
- Short cycled protocol (20-day courses)
- Derived from Khavinson bioregulation research
Possible side effects
- Injection site reactions
- May affect sleep timing if dosed incorrectly
- Limited Western safety data
- Bioregulator peptide
This list reflects published literature and community reports. Individual responses vary. Not medical advice.
Bloodwork worth tracking
The community-standard practice is a baseline panel before starting, a recheck 8 to 12 weeks in, and another after time off. For Pinealon, the markers most worth watching:
CMPCBCmelatonin levels
Typical time off: 2-3 mo between cycles
Lab panels are how a research protocol stays honest: numbers move before symptoms do. Interpretation belongs with a clinician. Not medical advice.
Lifestyle factors
None of the literature above was run on a background of poor sleep and no protein. These variables move outcomes more reliably than any compound on this site.
- Protein in the 1.6 to 2.2 g/kg range preserves lean mass through any body-composition phase.
- Resistance training 2 to 4 days per week plus 150+ minutes of weekly aerobic work reinforces whatever the protocol is doing.
- Sleep is the recovery window: 7 to 9 hours at consistent times.
- Hydration matters more than usual on compounds with GI or flushing effects: 2 to 3 liters daily.
- Track the variables that matter: weigh-ins, measurements, photos, and labs at sensible intervals.
Subcutaneous technique and practical notes
General best practice from clinical injection guidance — the same fundamentals nurses are taught, none of it specific to any compound.
References
- Nazimko et al., Adv Gerontol 2012 — oral 100 mcg twice daily in locomotive crews (English abstract only)
- Meshchaninov et al. 2015 — prooxidant activity and CD34+ decline in an older cohort
- Kozina 2008 — short peptides showed no direct antioxidant activity and raised intracellular ROS
- Mendzheritskii et al. 2011 — behavioural decrements and raised caspase-3 after carotid occlusion in rats
- Mendzheritsky et al. 2015 — Cortexin compared with Pinealon on free-radical processes
- PubMed — Pinealon literature
Quick answers
How many syringe units is a 2 mg dose of Pinealon?
At the standard reconstitution of 10 mg with 1 mL bacteriostatic water (10 mg/mL), a 2 mg dose draws 20 units (0.2 mL) on a U-100 insulin syringe.
How do you reconstitute Pinealon?
Add 1 mL of bacteriostatic water to the 10 mg vial for 10 mg/mL. Inject the water slowly down the inside wall of the vial, swirl gently without shaking, and refrigerate at 2 to 8 °C after mixing.
How long does a 10 mg vial of Pinealon last?
About 5 maintenance doses of 2 mg, roughly 0.7 weeks at once daily subcutaneous, morning.
Does Pinealon have human trial data?
No published dosing literature exists for Pinealon. The amounts shown are community convention only.
How does Pinealon work?
A tripeptide from the same Khavinson bioregulator series, associated in that literature with brain tissue and studied in rodent models of hypoxia and oxidative stress. Cell work reports that a labelled version enters the nucleus and associates with particular DNA sequences, which is the basis of the group’s claim that it acts directly on gene expression — though that association rests on fluorescence-quenching measurements rather than a direct binding assay.
Sourcing Pinealon
Whatever you are working with, the documentation matters more than the price. Ask for a batch certificate of analysis whose lot number matches the vial you actually receive — a COA for a different batch tells you nothing about yours.
Pinealon at Summit Research Supply → Grade a COA first
Summit is the supplier we feature and an affiliate link. Our COA checklist applies the same way to any vendor.
