NAD+ (500 mg vial)
Once daily subcutaneous protocol for longevity and cellular health research.
NAD+ is the central redox coenzyme of cellular metabolism, and injectable protocols aim to restore levels that decline with age. It ships as a pre-mixed solution, and the injections are higher volume than peptide draws. The protocol below reflects common subcutaneous practice.
Quick answer: at the standard 500 mg + 3 mL reconstitution, a 100 mg dose of NAD+ draws 60 units (0.6 mL) on a U-100 insulin syringe, once daily subcutaneous.
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Protocol overview
Dosing & reconstitution
Frequency: Once daily subcutaneous
Typical duration: 8-16 weeks
Reconstitution: 500 mg vial + 3 mL bacteriostatic water = 166.67 mg/mL. Units below are for a U-100 insulin syringe at this concentration.
| Phase | Dose | Units per injection |
|---|---|---|
| Week 1 | 50 mg | 30 units (0.3 mL) |
| Week 2 | 75 mg | 45 units (0.45 mL) |
| Weeks 3-16 | 100 mg | 60 units (0.6 mL) |
Units = (dose ÷ concentration) × 100 on a U-100 syringe. Change the vial size or water volume and the calculator redoes all of this for you.
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NAD+ dosing schedule
From a pharmacokinetic study of intravenous NAD+ in healthy men, which is the clearest published human dosing for infused NAD+ itself. Note it was designed to measure what happens to the molecule, not to demonstrate a clinical benefit.
| Phase | Amount | Frequency | Notes |
|---|---|---|---|
| Pilot study | 750 mg | Single infusion over 6 hours, intravenous | The amount and rate used in the published human PK study. |
Study length: Single 6-hour infusion in the pilot study.
Reconstitution and measurement
This part is arithmetic and does not depend on the evidence level. Concentration is vial size divided by the bacteriostatic water you add. Draw volume is your target amount divided by that concentration. Units are draw volume times 100, because a U-100 syringe reads 100 units per millilitre. Common vials for NAD+: 500 mg.
- Wipe the stoppers of both vials with an alcohol swab and let them dry.
- Draw the bacteriostatic water with a sterile syringe and inject it slowly down the inside wall of the peptide vial — never straight onto the powder.
- Swirl gently until the solution is clear. Do not shake; foaming and agitation damage peptides.
- Label the vial with the date and concentration, and refrigerate at 2–8 °C protected from light.
Worked example at the calculator defaults: 100 mg + 2 mL of bacteriostatic water = 50 mg/mL, so on a U-100 insulin syringe 1 unit = 500 mcg of NAD+. Change either input and the calculator redoes this for you.
Storage and stability
Supplies math
Whatever amounts your research uses, the planning arithmetic is the same. The calculator runs all of this per compound.
Supplies by protocol length
Based on the maintenance dose above (100 mg, once daily subcutaneous) and a 500 mg vial reconstituted with 3 mL.
| Duration | Injections | Vials | Syringes | Bac water | Swabs |
|---|---|---|---|---|---|
| 8 weeks | 56 | 12 x 500 mg | 62 | 4 x 10 mL | 112 |
| 12 weeks | 84 | 17 x 500 mg | 93 | 6 x 10 mL | 168 |
| 16 weeks | 112 | 23 x 500 mg | 124 | 7 x 10 mL | 224 |
Syringe count includes ~10% spares. Swabs = 2 per injection (stopper + site). Vial count assumes the full maintenance dose for the entire duration.
Important notes
Practical considerations for consistency and safety.
- Use a new sterile insulin syringe for each injection and dispose in a proper sharps container.
- Inject slowly and steadily; wait a few seconds before withdrawing the needle to ensure complete delivery.
- Administer at the same time each day for consistent levels. Setting a daily reminder helps maintain the schedule.
- The 500 mg vial size is standard for this compound. Reconstitute with 3 mL bacteriostatic water for a convenient concentration.
How NAD+ works
A coenzyme central to redox reactions and a substrate for sirtuins and PARPs. Tissue NAD+ falls with age, which is the basis of interest in restoring it — though whether infused NAD+ enters cells intact or is broken down first and salvaged is genuinely contested.
Benefits & side effects
Potential benefits
- Essential coenzyme for cellular energy metabolism
- Supports DNA repair and sirtuin activation
- Declines with age, supplementation restores levels
- May support healthy aging processes
Possible side effects
- Injection site pain and flushing (common)
- Nausea with larger doses
- High volume injections uncomfortable
- Flushing is a known NAD+ side effect
This list reflects published literature and community reports. Individual responses vary. Not medical advice.
Bloodwork worth tracking
The community-standard practice is a baseline panel before starting, a recheck 8 to 12 weeks in, and another after time off. For NAD+, the markers most worth watching:
CMPCBCNAD+ levels if available
Typical time off: 4-8 wks
Lab panels are how a research protocol stays honest: numbers move before symptoms do. Interpretation belongs with a clinician. Not medical advice.
Lifestyle factors
None of the literature above was run on a background of poor sleep and no protein. These variables move outcomes more reliably than any compound on this site.
- Protein in the 1.6 to 2.2 g/kg range preserves lean mass through any body-composition phase.
- Resistance training 2 to 4 days per week plus 150+ minutes of weekly aerobic work reinforces whatever the protocol is doing.
- Sleep is the recovery window: 7 to 9 hours at consistent times.
- Hydration matters more than usual on compounds with GI or flushing effects: 2 to 3 liters daily.
- Track the variables that matter: weigh-ins, measurements, photos, and labs at sensible intervals.
Subcutaneous technique and practical notes
General best practice from clinical injection guidance — the same fundamentals nurses are taught, none of it specific to any compound.
Quick answers
How many syringe units is a 100 mg dose of NAD+?
At the standard reconstitution of 500 mg with 3 mL bacteriostatic water (166.67 mg/mL), a 100 mg dose draws 60 units (0.6 mL) on a U-100 insulin syringe.
How do you reconstitute NAD+?
Add 3 mL of bacteriostatic water to the 500 mg vial for 166.67 mg/mL. Inject the water slowly down the inside wall of the vial, swirl gently without shaking, and refrigerate at 2 to 8 °C after mixing.
How long does a 500 mg vial of NAD+ last?
About 5 maintenance doses of 100 mg, roughly 0.7 weeks at once daily subcutaneous.
Does NAD+ have human trial data?
Yes. The dosing on this page is anchored to published human research (Grant R et al., "A Pilot Study Investigating Changes in the Human Plasma and Urine NAD+ Metabolome During a 6 Hour Intravenous Infusion of NAD+", Front Aging Neurosci 2019).
How does NAD+ work?
A coenzyme central to redox reactions and a substrate for sirtuins and PARPs. Tissue NAD+ falls with age, which is the basis of interest in restoring it — though whether infused NAD+ enters cells intact or is broken down first and salvaged is genuinely contested.
Sourcing NAD+
Whatever you are working with, the documentation matters more than the price. Ask for a batch certificate of analysis whose lot number matches the vial you actually receive — a COA for a different batch tells you nothing about yours.
NAD+ at Summit Research Supply → Grade a COA first
Summit is the supplier we feature and an affiliate link. Our COA checklist applies the same way to any vendor.
