Epitalon Reconstitution & Dosage Calculator
This free Epitalon calculator turns your vial size, the bacteriostatic water you add, and your target amount into a concentration, a draw volume, and the exact units on a U-100 insulin syringe. Epitalon (also written Epithalon) is a four-amino-acid peptide studied in longevity research, and it is typically handled in larger milligram amounts than most research peptides — so draws can approach a full syringe if you under-dilute. This tool is a measurement aid, not a protocol.
Epitalon reconstitution calculator
Syringe
U-100 insulinPeptide in vial
Target amount
Bacteriostatic water
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Quick summary
- Converts vial size (mg), bacteriostatic water (mL), and target amount into concentration, draw volume, and U-100 units.
- Reference math for the 10, 20, and 50 mg vials Epitalon is commonly supplied in.
- Flags the practical issue with Epitalon: milligram-scale amounts can exceed one syringe at low dilution.
What this Epitalon calculator does
This calculator does one job well: it turns your vial size, the amount of bacteriostatic water you add, and your target amount into a concentration (mg/mL), a draw volume (mL), and the matching units on a U-100 insulin syringe. Change any input and the result updates instantly.
Epitalon ships as a freeze-dried powder. Before it can be measured into a syringe it has to be reconstituted — dissolved in bacteriostatic water. How much water you add sets the concentration, and the concentration sets how many units each amount works out to. The presets above cover the most common Epitalon vial setups; use the custom fields for anything else.
How to use the Epitalon calculator
Pick your syringe
Choose the U-100 insulin syringe you'll draw with. Smaller syringes (0.3 mL / 30u) have finer lines, which helps when the draw is small.
Enter your vial and water
Set the milligrams in your Epitalon vial and the bacteriostatic water you added. Together these set the concentration.
Set your target amount
Toggle mg or mcg and pick (or type) the amount you're measuring for. The calculator does the conversion for you.
Read the draw
The result panel shows concentration, draw volume, and the exact U-100 units to pull, plus how many doses your vial contains.
Epitalon reconstitution math, explained
The math is short. Concentration = vial size ÷ bacteriostatic water. Draw volume = target amount ÷ concentration. Units = draw volume × 100 (a U-100 syringe reads 100 units per mL). The table below shows common Epitalon setups and the units for a 1 mg amount at each.
| Vial size | Bac water | Concentration | Units per 1 mg |
|---|---|---|---|
| 10 mg | 1.0 mL | 10 mg/mL | 10 units |
| 10 mg | 2.0 mL | 5 mg/mL | 20 units |
| 20 mg | 1.0 mL | 20 mg/mL | 5 units |
| 20 mg | 2.0 mL | 10 mg/mL | 10 units |
| 50 mg | 2.5 mL | 20 mg/mL | 5 units |
| 50 mg | 5.0 mL | 10 mg/mL | 10 units |
Epitalon amount-to-units reference
How common amounts convert to U-100 syringe units at two example concentrations. These are arithmetic conversions for reference, not a recommendation of any amount.
| Amount | Volume (mL) | U-100 units |
|---|---|---|
| 2.5 mg | 0.25 mL | 25 units |
| 5 mg | 0.50 mL | 50 units |
| 7.5 mg | 0.75 mL | 75 units |
| 10 mg | 1.00 mL | 100 units (a full U-100 syringe) |
| Amount | Volume (mL) | U-100 units |
|---|---|---|
| 2.5 mg | 0.125 mL | 12.5 units |
| 5 mg | 0.25 mL | 25 units |
| 7.5 mg | 0.375 mL | 37.5 units |
| 10 mg | 0.50 mL | 50 units |
Mixing, color & storage tips
Watch for draws over 100 units
A U-100 syringe holds 1 mL — exactly 100 units. Because Epitalon is handled in whole milligrams, a 10 mg target at 10 mg/mL fills the entire syringe. If your calculated draw exceeds 100 units, use less water (a higher concentration) so the amount fits in one draw.
Mixing without foam
Add the bacteriostatic water slowly down the inside wall of the vial rather than onto the powder directly, then swirl gently until dissolved. Do not shake.
Storage and vial life
Keep the dry powder cold and dark. After reconstitution, refrigerate, keep it out of light, and plan around a limited usable window. Do not freeze a reconstituted vial.
Epitalon vs Epithalon spelling
Epitalon, Epithalon, and Epithalamin all appear in the literature. Epitalon and Epithalon refer to the same synthetic AEDG tetrapeptide; Epithalamin refers to the earlier pineal extract it was derived from.
Epitalon supplies checklist
A simple reconstitution shopping list. Confirm vial size and batch documentation before you buy.

Epitalon
- Batch COA on every vial
- Third-party purity tested
- U.S. fulfillment, discreet shipping
Epitalon — frequently asked questions
What does this Epitalon calculator tell me?
It converts your vial size, bacteriostatic water volume, and target amount into concentration in mg/mL, draw volume in mL, and the matching units on a U-100 insulin syringe. It is a measurement tool, not a recommendation.
Is the Epitalon calculator free?
Yes. It runs entirely in your browser, is free, and requires no account or signup.
How much bacteriostatic water do I add to a 10 mg Epitalon vial?
That depends on the concentration you want. 10 mg with 1 mL makes 10 mg/mL, so a 5 mg target is 50 units. Using 2 mL makes 5 mg/mL, and that same 5 mg target becomes a full 100-unit syringe — which is why less water is often preferred for Epitalon.
How many units is 5 mg of Epitalon?
At 10 mg/mL it is 50 units (0.5 mL). At 20 mg/mL it is 25 units (0.25 mL). Units always depend on your specific concentration.
Why does my Epitalon draw exceed 100 units?
Because Epitalon is measured in whole milligrams, a large target at a low concentration can exceed the 1 mL a U-100 syringe holds. Reconstitute with less water to raise the concentration so the amount fits in a single draw.
Is Epitalon the same as Epithalon?
Yes. Both spellings refer to the same synthetic AEDG (Ala-Glu-Asp-Gly) tetrapeptide. Epithalamin is a different, earlier pineal-extract preparation.
Is Epitalon FDA-approved?
No. Epitalon is not an FDA-approved drug. It is sold strictly as a research-use-only compound, not for human or veterinary use.
Is this medical advice?
No. This page and calculator are for education and research planning only. They do not diagnose, treat, or recommend an amount.

