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Protocol Reference
IMMUNE

Thymalin (10 mg vial)

Once daily intramuscular protocol for immune research.

Thymalin is a thymic extract from the Soviet bioregulator program, used clinically in Russia for immune support, with short courses and long gaps as the traditional format. The protocol below follows that structure intramuscularly.

Quick answer: at the standard 10 mg + 1 mL reconstitution, a 10 mg dose of Thymalin draws 100 units (1.0 mL) on a U-100 insulin syringe, once daily intramuscular.

Get Thymalin → code PEPDOSE

Category
Immune & Thymic
Common vials
10 mg
Half-life
Not established
Route in the literature
Intramuscular in the published courses

Protocol overview

Goal
A polypeptide complex extracted from calf thymus, rather than a single defined peptide. It sits in the same Russian b...
Schedule
Once daily intramuscular, 10-day courses, repeat every 3-6 months
Reconstitution
10 mg vial + 1 mL bac water (10 mg/mL)
Storage
Lyophilized: frozen or 2-8 °C; reconstituted: refrigerated, use within 28 days
Evidence
There is no meaningful published dosing literature for this compound in humans.

Dosing & reconstitution

Community-reported ranges. These are the amounts commonly reported in user communities and vendor protocols, not from controlled human trials. They are listed here because they are what most people reference in practice.

Frequency: Once daily intramuscular
Typical duration: 10-day courses, repeat every 3-6 months

Reconstitution: 10 mg vial + 1 mL bacteriostatic water = 10 mg/mL. Units below are for a U-100 insulin syringe at this concentration.

PhaseDoseUnits per injection
Days 1-1010 mg100 units (1.0 mL)

Units = (dose ÷ concentration) × 100 on a U-100 syringe. Change the vial size or water volume and the calculator redoes all of this for you.

Track this protocol → Free account · log doses, rotate sites, never lose your history

Thymalin dosing — what the literature actually supports

No dosing literature. Thymalin has Russian clinical use and an associated Russian-language literature, including some long-term geriatric follow-up from the Khavinson group. But it is an undefined extract rather than a standardised molecule, independent replication is absent, and there is no trial we can cite that establishes a schedule to modern standards. Do not confuse it with Thymosin Alpha-1, which is a single defined peptide with an actual regulatory dossier.

We publish a schedule when there is a citable human source and we say so when there is not. Filling this space with a number nobody measured would make the page look more complete and be worth less than nothing to you.

Reconstitution and measurement

This part is arithmetic and does not depend on the evidence level. Concentration is vial size divided by the bacteriostatic water you add. Draw volume is your target amount divided by that concentration. Units are draw volume times 100, because a U-100 syringe reads 100 units per millilitre. Common vials for Thymalin: 10 mg.

  1. Wipe the stoppers of both vials with an alcohol swab and let them dry.
  2. Draw the bacteriostatic water with a sterile syringe and inject it slowly down the inside wall of the peptide vial — never straight onto the powder.
  3. Swirl gently until the solution is clear. Do not shake; foaming and agitation damage peptides.
  4. Label the vial with the date and concentration, and refrigerate at 2–8 °C protected from light.

Worked example at the calculator defaults: 10 mg + 2 mL of bacteriostatic water = 5 mg/mL, so on a U-100 insulin syringe 1 unit = 50 mcg of Thymalin. Change either input and the calculator redoes this for you.

Open the Thymalin calculator → Unit converter

Storage and stability

Dry powder
Cold, dark and dry. Lyophilized peptide is the stable form — keep it that way until you intend to use it.
After reconstitution
Refrigerate at 2–8 °C, protect from light, and plan around a limited window. Do not freeze a reconstituted vial — freeze-thaw damages peptides.
Mixing
Run bacteriostatic water down the inside wall of the vial rather than onto the powder, then swirl. Never shake.
Inspect before use
A clear solution is expected. Cloudiness or particles mean discard, not use.

Full storage guide → · Cloudy solution?

Supplies math

Whatever amounts your research uses, the planning arithmetic is the same. The calculator runs all of this per compound.

Vials
Doses per vial = vial size in mg divided by your per-dose amount in mg. The calculator on this page runs that arithmetic for any vial size and amount you enter.
Syringes
One sterile U-100 insulin syringe per injection, plus roughly 10% spares for bent tips and mis-draws. Daily protocols run 30 or 31 per month.
Bacteriostatic water
1 to 3 mL per vial reconstituted. A single 10 mL bottle covers 3 to 10 vials, so one bottle usually outlasts several vials.
Alcohol swabs
Two per injection: one for the vial stopper, one for the site. A 100-count box covers about 7 weeks of daily work.

Supplies by protocol length

Based on the maintenance dose above (10 mg, once daily intramuscular) and a 10 mg vial reconstituted with 1 mL.

DurationInjectionsVialsSyringesBac waterSwabs
8 weeks5656 x 10 mg626 x 10 mL112
12 weeks8484 x 10 mg939 x 10 mL168
16 weeks112112 x 10 mg12412 x 10 mL224

Syringe count includes ~10% spares. Swabs = 2 per injection (stopper + site). Vial count assumes the full maintenance dose for the entire duration.

Important notes

Practical considerations for consistency and safety.

  • Use a new sterile insulin syringe for each injection and dispose in a proper sharps container.
  • Rotate intramuscular injection sites (deltoid, vastus lateralis, gluteus medius) and use the appropriate needle gauge for the muscle.
  • Inject slowly and steadily; wait a few seconds before withdrawing the needle to ensure complete delivery.
  • Administer at the same time each day for consistent levels. Setting a daily reminder helps maintain the schedule.
  • This compound lacks controlled human safety data. Start with the lowest reported amount and increase gradually while monitoring for any adverse reactions.
  • The 10 mg vial size is standard for this compound. Reconstitute with 1 mL bacteriostatic water for a convenient concentration.

How Thymalin works

A polypeptide complex extracted from calf thymus, rather than a single defined peptide. It sits in the same Russian bioregulator tradition as the Khavinson compounds and is used there as an immune-restorative.

Benefits & side effects

Potential benefits

  • Thymic extract supporting immune function
  • Supports T-cell maturation and immune regulation
  • Short course protocol with long breaks
  • Used clinically in some countries

Possible side effects

  • Injection site reactions (IM)
  • Mild fever possible
  • Short courses with long breaks for safety
  • Limited Western clinical data

This list reflects published literature and community reports. Individual responses vary. Not medical advice.

Bloodwork worth tracking

The community-standard practice is a baseline panel before starting, a recheck 8 to 12 weeks in, and another after time off. For Thymalin, the markers most worth watching:

CBC with differentialCMPCRP

Typical time off: 3-6 mo between courses

Lab panels are how a research protocol stays honest: numbers move before symptoms do. Interpretation belongs with a clinician. Not medical advice.

Lifestyle factors

None of the literature above was run on a background of poor sleep and no protein. These variables move outcomes more reliably than any compound on this site.

  • Protein in the 1.6 to 2.2 g/kg range preserves lean mass through any body-composition phase.
  • Resistance training 2 to 4 days per week plus 150+ minutes of weekly aerobic work reinforces whatever the protocol is doing.
  • Sleep is the recovery window: 7 to 9 hours at consistent times.
  • Hydration matters more than usual on compounds with GI or flushing effects: 2 to 3 liters daily.
  • Track the variables that matter: weigh-ins, measurements, photos, and labs at sensible intervals.

Subcutaneous technique and practical notes

General best practice from clinical injection guidance — the same fundamentals nurses are taught, none of it specific to any compound.

Fresh sterile syringe, every time
Reusing needles dulls the tip, hurts more, and is the easiest contamination route. Dispose of used syringes in a sharps container, not the bin.
Rotate sites
Abdomen (5 cm clear of the navel), thighs, upper arms. Rotating systematically prevents localised irritation and lipohypertrophy.
Slow and steady
Pinch a skinfold, insert at 45–90°, inject slowly, and wait a few seconds before withdrawing. Subcutaneous injections are not aspirated.
Write it down
Log the date, amount and site. A record is the only way to keep any research protocol consistent — and the first thing to review if anything looks off.

Quick answers

How many syringe units is a 10 mg dose of Thymalin?

At the standard reconstitution of 10 mg with 1 mL bacteriostatic water (10 mg/mL), a 10 mg dose draws 100 units (1.0 mL) on a U-100 insulin syringe.

How do you reconstitute Thymalin?

Add 1 mL of bacteriostatic water to the 10 mg vial for 10 mg/mL. Inject the water slowly down the inside wall of the vial, swirl gently without shaking, and refrigerate at 2 to 8 °C after mixing.

How long does a 10 mg vial of Thymalin last?

About 1 maintenance doses of 10 mg, roughly 0.1 weeks at once daily intramuscular.

Does Thymalin have human trial data?

No published dosing literature exists for Thymalin. The amounts shown are community convention only.

How does Thymalin work?

A polypeptide complex extracted from calf thymus, rather than a single defined peptide. It sits in the same Russian bioregulator tradition as the Khavinson compounds and is used there as an immune-restorative.

Sourcing Thymalin

Whatever you are working with, the documentation matters more than the price. Ask for a batch certificate of analysis whose lot number matches the vial you actually receive — a COA for a different batch tells you nothing about yours.

Thymalin at Summit Research Supply → Grade a COA first

Summit is the supplier we feature and an affiliate link. Our COA checklist applies the same way to any vendor.

For laboratory research use only. This page documents what the published literature reports about Thymalin. It is not a recommendation, not a treatment plan, and not medical advice. Compounds referenced are sold strictly as research chemicals and are not for human or veterinary use. Some supplier links are affiliate links and may earn us a commission. This never affects tier placement or review conclusions.
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