TB-500 (10 mg vial)
Once daily subcutaneous protocol for tissue-repair research.
TB-500 is the synthetic version of thymosin beta-4’s active fragment, an actin-regulating peptide studied preclinically for cell migration and tissue repair. It acts systemically, so injection site matters little. The protocol below runs a loading phase then maintenance, subcutaneous.
Quick answer: at the standard 10 mg + 1 mL reconstitution, a 1 mg dose of TB-500 draws 10 units (0.1 mL) on a U-100 insulin syringe, once daily subcutaneous.
Get TB-500 → code PEPDOSE
Protocol overview
Dosing & reconstitution
Frequency: Once daily subcutaneous
Typical duration: 8-12 weeks
Reconstitution: 10 mg vial + 1 mL bacteriostatic water = 10 mg/mL. Units below are for a U-100 insulin syringe at this concentration.
| Phase | Dose | Units per injection |
|---|---|---|
| Weeks 1-2 | 500 mcg | 5 units (0.05 mL) |
| Weeks 3-4 | 600 mcg | 6 units (0.06 mL) |
| Weeks 5-8 | 750 mcg | 7.5 units (0.075 mL) |
| Weeks 9-12 | 1 mg | 10 units (0.1 mL) |
Units = (dose ÷ concentration) × 100 on a U-100 syringe. Change the vial size or water volume and the calculator redoes all of this for you.
Track this protocol → Free account · log doses, rotate sites, never lose your history
TB-500 dosing — what the literature actually supports
We publish a schedule when there is a citable human source and we say so when there is not. Filling this space with a number nobody measured would make the page look more complete and be worth less than nothing to you.
Reconstitution and measurement
This part is arithmetic and does not depend on the evidence level. Concentration is vial size divided by the bacteriostatic water you add. Draw volume is your target amount divided by that concentration. Units are draw volume times 100, because a U-100 syringe reads 100 units per millilitre. Common vials for TB-500: 10 mg.
- Wipe the stoppers of both vials with an alcohol swab and let them dry.
- Draw the bacteriostatic water with a sterile syringe and inject it slowly down the inside wall of the peptide vial — never straight onto the powder.
- Swirl gently until the solution is clear. Do not shake; foaming and agitation damage peptides.
- Label the vial with the date and concentration, and refrigerate at 2–8 °C protected from light.
Worked example at the calculator defaults: 5 mg + 2 mL of bacteriostatic water = 2.5 mg/mL, so on a U-100 insulin syringe 1 unit = 25 mcg of TB-500. Change either input and the calculator redoes this for you.
Storage and stability
Supplies math
Whatever amounts your research uses, the planning arithmetic is the same. The calculator runs all of this per compound.
Supplies by protocol length
Based on the maintenance dose above (1 mg, once daily subcutaneous) and a 10 mg vial reconstituted with 1 mL.
| Duration | Injections | Vials | Syringes | Bac water | Swabs |
|---|---|---|---|---|---|
| 8 weeks | 56 | 6 x 10 mg | 62 | 1 x 10 mL | 112 |
| 12 weeks | 84 | 9 x 10 mg | 93 | 1 x 10 mL | 168 |
| 16 weeks | 112 | 12 x 10 mg | 124 | 2 x 10 mL | 224 |
Syringe count includes ~10% spares. Swabs = 2 per injection (stopper + site). Vial count assumes the full maintenance dose for the entire duration.
Important notes
Practical considerations for consistency and safety.
- Use a new sterile insulin syringe for each injection and dispose in a proper sharps container.
- Rotate injection sites (abdomen, thighs, upper arms) systematically to reduce local irritation and avoid lipohypertrophy.
- Rotate intramuscular injection sites (deltoid, vastus lateralis, gluteus medius) and use the appropriate needle gauge for the muscle.
- Inject slowly and steadily; wait a few seconds before withdrawing the needle to ensure complete delivery.
- Administer at the same time each day for consistent levels. Setting a daily reminder helps maintain the schedule.
- This compound lacks controlled human safety data. Start with the lowest reported amount and increase gradually while monitoring for any adverse reactions.
- The 10 mg vial size is standard for this compound. Reconstitute with 1 mL bacteriostatic water for a convenient concentration.
How TB-500 works
A synthetic fragment of thymosin beta-4, the actin-sequestering protein. The parent protein has been studied in human trials for wound healing and dry eye; TB-500 as sold is the fragment, not the full protein.
Benefits & side effects
Potential benefits
- Promotes cell migration and tissue repair in preclinical models
- Anti-inflammatory properties reported in animal work
- Systemic action, not limited to injection site
- Supports wound healing and recovery in models
Possible side effects
- No human clinical safety data
- Injection site reactions possible
- Theoretical concern about promoting growth in existing tumours
- All evidence is preclinical
This list reflects published literature and community reports. Individual responses vary. Not medical advice.
Bloodwork worth tracking
The community-standard practice is a baseline panel before starting, a recheck 8 to 12 weeks in, and another after time off. For TB-500, the markers most worth watching:
CMPCBC
Typical time off: 2-4 wks
Lab panels are how a research protocol stays honest: numbers move before symptoms do. Interpretation belongs with a clinician. Not medical advice.
Lifestyle factors
None of the literature above was run on a background of poor sleep and no protein. These variables move outcomes more reliably than any compound on this site.
- Protein in the 1.6 to 2.2 g/kg range preserves lean mass through any body-composition phase.
- Resistance training 2 to 4 days per week plus 150+ minutes of weekly aerobic work reinforces whatever the protocol is doing.
- Sleep is the recovery window: 7 to 9 hours at consistent times.
- Hydration matters more than usual on compounds with GI or flushing effects: 2 to 3 liters daily.
- Track the variables that matter: weigh-ins, measurements, photos, and labs at sensible intervals.
Subcutaneous technique and practical notes
General best practice from clinical injection guidance — the same fundamentals nurses are taught, none of it specific to any compound.
Quick answers
How many syringe units is a 1 mg dose of TB-500?
At the standard reconstitution of 10 mg with 1 mL bacteriostatic water (10 mg/mL), a 1 mg dose draws 10 units (0.1 mL) on a U-100 insulin syringe.
How do you reconstitute TB-500?
Add 1 mL of bacteriostatic water to the 10 mg vial for 10 mg/mL. Inject the water slowly down the inside wall of the vial, swirl gently without shaking, and refrigerate at 2 to 8 °C after mixing.
How long does a 10 mg vial of TB-500 last?
About 10 maintenance doses of 1 mg, roughly 1.4 weeks at once daily subcutaneous.
Does TB-500 have human trial data?
No. The evidence base is animal and cell studies; the practical dosing shown is community convention, and the page labels it that way.
How does TB-500 work?
A synthetic fragment of thymosin beta-4, the actin-sequestering protein. The parent protein has been studied in human trials for wound healing and dry eye; TB-500 as sold is the fragment, not the full protein.
Sourcing TB-500
Whatever you are working with, the documentation matters more than the price. Ask for a batch certificate of analysis whose lot number matches the vial you actually receive — a COA for a different batch tells you nothing about yours.
TB-500 at Summit Research Supply → Grade a COA first
Summit is the supplier we feature and an affiliate link. Our COA checklist applies the same way to any vendor.
