DSIP (5 mg vial)
Once daily subcutaneous, evening protocol for cognitive and neurological research.
Delta sleep-inducing peptide is one of the oldest compounds in this library, first isolated in the 1970s from studies of sleep architecture. Evidence never matured past small mixed studies. The protocol below is evening subcutaneous dosing before bed.
Quick answer: at the standard 5 mg + 0.5 mL reconstitution, a 250 mcg dose of DSIP draws 2.5 units (0.025 mL) on a U-100 insulin syringe, once daily subcutaneous, evening.
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Protocol overview
Dosing & reconstitution
Frequency: Once daily subcutaneous, evening
Typical duration: 4-8 weeks
Reconstitution: 5 mg vial + 0.5 mL bacteriostatic water = 10 mg/mL. Units below are for a U-100 insulin syringe at this concentration.
| Phase | Dose | Units per injection |
|---|---|---|
| Week 1 | 100 mcg | 1 unit (0.01 mL) |
| Week 2 | 150 mcg | 1.5 units (0.015 mL) |
| Week 3 | 200 mcg | 2 units (0.02 mL) |
| Weeks 4-8 | 250 mcg | 2.5 units (0.025 mL) |
Units = (dose ÷ concentration) × 100 on a U-100 syringe. Change the vial size or water volume and the calculator redoes all of this for you.
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DSIP dosing — what the literature actually supports
We publish a schedule when there is a citable human source and we say so when there is not. Filling this space with a number nobody measured would make the page look more complete and be worth less than nothing to you.
Reconstitution and measurement
This part is arithmetic and does not depend on the evidence level. Concentration is vial size divided by the bacteriostatic water you add. Draw volume is your target amount divided by that concentration. Units are draw volume times 100, because a U-100 syringe reads 100 units per millilitre. Common vials for DSIP: 5 mg.
- Wipe the stoppers of both vials with an alcohol swab and let them dry.
- Draw the bacteriostatic water with a sterile syringe and inject it slowly down the inside wall of the peptide vial — never straight onto the powder.
- Swirl gently until the solution is clear. Do not shake; foaming and agitation damage peptides.
- Label the vial with the date and concentration, and refrigerate at 2–8 °C protected from light.
Worked example at the calculator defaults: 5 mg + 2 mL of bacteriostatic water = 2.5 mg/mL, so on a U-100 insulin syringe 1 unit = 25 mcg of DSIP. Change either input and the calculator redoes this for you.
Storage and stability
Supplies math
Whatever amounts your research uses, the planning arithmetic is the same. The calculator runs all of this per compound.
Supplies by protocol length
Based on the maintenance dose above (250 mcg, once daily subcutaneous, evening) and a 5 mg vial reconstituted with 0.5 mL.
| Duration | Injections | Vials | Syringes | Bac water | Swabs |
|---|---|---|---|---|---|
| 8 weeks | 56 | 3 x 5 mg | 62 | 1 x 10 mL | 112 |
| 12 weeks | 84 | 5 x 5 mg | 93 | 1 x 10 mL | 168 |
| 16 weeks | 112 | 6 x 5 mg | 124 | 1 x 10 mL | 224 |
Syringe count includes ~10% spares. Swabs = 2 per injection (stopper + site). Vial count assumes the full maintenance dose for the entire duration.
Important notes
Practical considerations for consistency and safety.
- Use a new sterile insulin syringe for each injection and dispose in a proper sharps container.
- Inject slowly and steadily; wait a few seconds before withdrawing the needle to ensure complete delivery.
- Administer at the same time each day for consistent levels. Setting a daily reminder helps maintain the schedule.
- This compound lacks controlled human safety data. Start with the lowest reported amount and increase gradually while monitoring for any adverse reactions.
- The 5 mg vial size is standard for this compound. Reconstitute with 0.5 mL bacteriostatic water for a convenient concentration.
How DSIP works
A nonapeptide originally isolated from the cerebral venous blood of rabbits in induced sleep, and named for the delta-wave activity observed. Its endogenous role in humans has never been firmly established.
Benefits & side effects
Potential benefits
- Delta sleep-inducing peptide for sleep quality
- Supports natural sleep architecture
- May help normalize disrupted sleep patterns
- Evening dosing aligns with circadian rhythm
Possible side effects
- Injection site reactions
- Excessive drowsiness if dose too high
- Headache occasionally
- Limited long-term safety data
This list reflects published literature and community reports. Individual responses vary. Not medical advice.
Bloodwork worth tracking
The community-standard practice is a baseline panel before starting, a recheck 8 to 12 weeks in, and another after time off. For DSIP, the markers most worth watching:
CMPCBCcortisol
Typical time off: 4 wks
Lab panels are how a research protocol stays honest: numbers move before symptoms do. Interpretation belongs with a clinician. Not medical advice.
Lifestyle factors
None of the literature above was run on a background of poor sleep and no protein. These variables move outcomes more reliably than any compound on this site.
- Protein in the 1.6 to 2.2 g/kg range preserves lean mass through any body-composition phase.
- Resistance training 2 to 4 days per week plus 150+ minutes of weekly aerobic work reinforces whatever the protocol is doing.
- Sleep is the recovery window: 7 to 9 hours at consistent times.
- Hydration matters more than usual on compounds with GI or flushing effects: 2 to 3 liters daily.
- Track the variables that matter: weigh-ins, measurements, photos, and labs at sensible intervals.
Subcutaneous technique and practical notes
General best practice from clinical injection guidance — the same fundamentals nurses are taught, none of it specific to any compound.
Quick answers
How many syringe units is a 250 mcg dose of DSIP?
At the standard reconstitution of 5 mg with 0.5 mL bacteriostatic water (10 mg/mL), a 250 mcg dose draws 2.5 units (0.025 mL) on a U-100 insulin syringe.
How do you reconstitute DSIP?
Add 0.5 mL of bacteriostatic water to the 5 mg vial for 10 mg/mL. Inject the water slowly down the inside wall of the vial, swirl gently without shaking, and refrigerate at 2 to 8 °C after mixing.
How long does a 5 mg vial of DSIP last?
About 20 maintenance doses of 250 mcg, roughly 2.9 weeks at once daily subcutaneous, evening.
Does DSIP have human trial data?
No. The evidence base is animal and cell studies; the practical dosing shown is community convention, and the page labels it that way.
How does DSIP work?
A nonapeptide originally isolated from the cerebral venous blood of rabbits in induced sleep, and named for the delta-wave activity observed. Its endogenous role in humans has never been firmly established.
Sourcing DSIP
Whatever you are working with, the documentation matters more than the price. Ask for a batch certificate of analysis whose lot number matches the vial you actually receive — a COA for a different batch tells you nothing about yours.
DSIP at Summit Research Supply → Grade a COA first
Summit is the supplier we feature and an affiliate link. Our COA checklist applies the same way to any vendor.
