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Protocol Reference
GROWTH HORMONE

CJC-1295 + Ipamorelin (10 mg vial)

Once daily subcutaneous protocol for growth hormone axis research.

This is the standard GH secretagogue stack: CJC-1295 without DAC raises the amplitude of the natural GH pulse while ipamorelin triggers the release, from two different receptors. The protocol below is one bedtime subcutaneous injection, dose referring to each component.

Quick answer: at the standard 10 mg + 2 mL reconstitution, a 250 mcg each dose of CJC-1295 + Ipamorelin draws 10 units (0.1 mL) on a U-100 insulin syringe, once daily subcutaneous.

Get CJC-1295 + Ipamorelin → code PEPDOSE

Category
Growth Hormone Axis
Common vials
10 mg total (5/5)
Half-life
~30 min (CJC no-DAC) / ~2 h (ipamorelin)
Route in the literature
Subcutaneous

Protocol overview

Goal
Pairs a GHRH analog with a ghrelin-receptor agonist. The rationale is that the two act on different receptors, so the...
Schedule
Once daily subcutaneous, 8-12 weeks
Reconstitution
10 mg vial + 2 mL bac water (5 mg/mL)
Storage
Lyophilized: frozen or 2-8 °C; reconstituted: refrigerated, use within 28 days
Evidence
The published work on this compound is animal or cell-culture research. No human dosing schedule exists to cite.

Dosing & reconstitution

Community-reported ranges. These are the amounts commonly reported in user communities and vendor protocols, not from controlled human trials. They are listed here because they are what most people reference in practice.

Frequency: Once daily subcutaneous
Typical duration: 8-12 weeks

Reconstitution: 10 mg vial + 2 mL bacteriostatic water = 5 mg/mL. Units below are for a U-100 insulin syringe at this concentration.

PhaseDoseUnits per injection
Weeks 1-2100 mcg each4 units (0.04 mL)
Weeks 3-4150 mcg each6 units (0.06 mL)
Weeks 5-6200 mcg each8 units (0.08 mL)
Weeks 7-12250 mcg each10 units (0.1 mL)

Units = (dose ÷ concentration) × 100 on a U-100 syringe. Change the vial size or water volume and the calculator redoes all of this for you.

Track this protocol → Free account · log doses, rotate sites, never lose your history

CJC-1295 + Ipamorelin dosing — what the literature actually supports

Animal data only. The individual components have human pharmacology data, but this specific combination has not been studied in a human trial. There is no citable schedule for the blend, and combining two single-agent schedules is an assumption rather than a finding. Confusing the no-DAC form with CJC-1295 with DAC is also a common and consequential error — their half-lives differ by roughly two orders of magnitude.

We publish a schedule when there is a citable human source and we say so when there is not. Filling this space with a number nobody measured would make the page look more complete and be worth less than nothing to you.

Reconstitution and measurement

This part is arithmetic and does not depend on the evidence level. Concentration is vial size divided by the bacteriostatic water you add. Draw volume is your target amount divided by that concentration. Units are draw volume times 100, because a U-100 syringe reads 100 units per millilitre. Common vials for CJC-1295 + Ipamorelin: 10 mg total (5/5).

  1. Wipe the stoppers of both vials with an alcohol swab and let them dry.
  2. Draw the bacteriostatic water with a sterile syringe and inject it slowly down the inside wall of the peptide vial — never straight onto the powder.
  3. Swirl gently until the solution is clear. Do not shake; foaming and agitation damage peptides.
  4. Label the vial with the date and concentration, and refrigerate at 2–8 °C protected from light.

Open the CJC-1295 + Ipamorelin calculator → Unit converter

Storage and stability

Dry powder
Cold, dark and dry. Lyophilized peptide is the stable form — keep it that way until you intend to use it.
After reconstitution
Refrigerate at 2–8 °C, protect from light, and plan around a limited window. Do not freeze a reconstituted vial — freeze-thaw damages peptides.
Mixing
Run bacteriostatic water down the inside wall of the vial rather than onto the powder, then swirl. Never shake.
Inspect before use
A clear solution is expected. Cloudiness or particles mean discard, not use.

Full storage guide → · Cloudy solution?

Supplies math

Whatever amounts your research uses, the planning arithmetic is the same. The calculator runs all of this per compound.

Vials
Doses per vial = vial size in mg divided by your per-dose amount in mg. The calculator on this page runs that arithmetic for any vial size and amount you enter.
Syringes
One sterile U-100 insulin syringe per injection, plus roughly 10% spares for bent tips and mis-draws. Daily protocols run 30 or 31 per month.
Bacteriostatic water
1 to 3 mL per vial reconstituted. A single 10 mL bottle covers 3 to 10 vials, so one bottle usually outlasts several vials.
Alcohol swabs
Two per injection: one for the vial stopper, one for the site. A 100-count box covers about 7 weeks of daily work.

Supplies by protocol length

Based on the maintenance dose above (250 mcg each, once daily subcutaneous) and a 10 mg vial reconstituted with 2 mL.

DurationInjectionsVialsSyringesBac waterSwabs
8 weeks562 x 10 mg621 x 10 mL112
12 weeks843 x 10 mg931 x 10 mL168
16 weeks1123 x 10 mg1241 x 10 mL224

Syringe count includes ~10% spares. Swabs = 2 per injection (stopper + site). Vial count assumes the full maintenance dose for the entire duration.

Important notes

Practical considerations for consistency and safety.

  • Use a new sterile insulin syringe for each injection and dispose in a proper sharps container.
  • Rotate injection sites (abdomen, thighs, upper arms) systematically to reduce local irritation and avoid lipohypertrophy.
  • Inject slowly and steadily; wait a few seconds before withdrawing the needle to ensure complete delivery.
  • Administer at the same time each day for consistent levels. Setting a daily reminder helps maintain the schedule.
  • This compound lacks controlled human safety data. Start with the lowest reported amount and increase gradually while monitoring for any adverse reactions.
  • The 10 mg vial size is standard for this compound. Reconstitute with 2 mL bacteriostatic water for a convenient concentration.

How CJC-1295 + Ipamorelin works

Pairs a GHRH analog with a ghrelin-receptor agonist. The rationale is that the two act on different receptors, so the combination is expected to produce a larger GH pulse than either alone. Note the CJC here is the no-DAC form, with a half-life of minutes rather than days.

Benefits & side effects

Potential benefits

  • Synergistic GH release from dual pathways
  • Preserves natural pulsatile GH pattern
  • Well-tolerated combination
  • Popular GH secretagogue stack

Possible side effects

  • Injection site reactions
  • Facial flushing from CJC component
  • Headache occasionally
  • Water retention possible

This list reflects published literature and community reports. Individual responses vary. Not medical advice.

Bloodwork worth tracking

The community-standard practice is a baseline panel before starting, a recheck 8 to 12 weeks in, and another after time off. For CJC-1295 + Ipamorelin, the markers most worth watching:

IGF-1fasting glucoselipid panel

Typical time off: 4-8 wks

Lab panels are how a research protocol stays honest: numbers move before symptoms do. Interpretation belongs with a clinician. Not medical advice.

Lifestyle factors

None of the literature above was run on a background of poor sleep and no protein. These variables move outcomes more reliably than any compound on this site.

  • Protein in the 1.6 to 2.2 g/kg range preserves lean mass through any body-composition phase.
  • Resistance training 2 to 4 days per week plus 150+ minutes of weekly aerobic work reinforces whatever the protocol is doing.
  • Sleep is the recovery window: 7 to 9 hours at consistent times.
  • Hydration matters more than usual on compounds with GI or flushing effects: 2 to 3 liters daily.
  • Track the variables that matter: weigh-ins, measurements, photos, and labs at sensible intervals.

Subcutaneous technique and practical notes

General best practice from clinical injection guidance — the same fundamentals nurses are taught, none of it specific to any compound.

Fresh sterile syringe, every time
Reusing needles dulls the tip, hurts more, and is the easiest contamination route. Dispose of used syringes in a sharps container, not the bin.
Rotate sites
Abdomen (5 cm clear of the navel), thighs, upper arms. Rotating systematically prevents localised irritation and lipohypertrophy.
Slow and steady
Pinch a skinfold, insert at 45–90°, inject slowly, and wait a few seconds before withdrawing. Subcutaneous injections are not aspirated.
Write it down
Log the date, amount and site. A record is the only way to keep any research protocol consistent — and the first thing to review if anything looks off.

Quick answers

How many syringe units is a 250 mcg each dose of CJC-1295 + Ipamorelin?

At the standard reconstitution of 10 mg with 2 mL bacteriostatic water (5 mg/mL), a 250 mcg each dose draws 10 units (0.1 mL) on a U-100 insulin syringe.

How do you reconstitute CJC-1295 + Ipamorelin?

Add 2 mL of bacteriostatic water to the 10 mg vial for 5 mg/mL. Inject the water slowly down the inside wall of the vial, swirl gently without shaking, and refrigerate at 2 to 8 °C after mixing.

How long does a 10 mg vial of CJC-1295 + Ipamorelin last?

About 40 maintenance doses of 250 mcg each, roughly 5.7 weeks at once daily subcutaneous.

Does CJC-1295 + Ipamorelin have human trial data?

No. The evidence base is animal and cell studies; the practical dosing shown is community convention, and the page labels it that way.

How does CJC-1295 + Ipamorelin work?

Pairs a GHRH analog with a ghrelin-receptor agonist. The rationale is that the two act on different receptors, so the combination is expected to produce a larger GH pulse than either alone. Note the CJC here is the no-DAC form, with a half-life of minutes rather than days.

Sourcing CJC-1295 + Ipamorelin

Whatever you are working with, the documentation matters more than the price. Ask for a batch certificate of analysis whose lot number matches the vial you actually receive — a COA for a different batch tells you nothing about yours.

CJC-1295/Ipamorelin Blend at Summit Research Supply → Grade a COA first

Summit is the supplier we feature and an affiliate link. Our COA checklist applies the same way to any vendor.

For laboratory research use only. This page documents what the published literature reports about CJC-1295 + Ipamorelin. It is not a recommendation, not a treatment plan, and not medical advice. Compounds referenced are sold strictly as research chemicals and are not for human or veterinary use. Some supplier links are affiliate links and may earn us a commission. This never affects tier placement or review conclusions.
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